Effect of Complex Prebiotics on the Intestinal Colonization Ability of Limosilactobacillus fermentum DALI02

Intestinal colonization is beneficial to the role of probiotics, and prebiotics can promote adhesion probiotics in intestine. This study optimized combination complex that could improve growth ability Limosilactobacillus fermentum (L. fermentum) DALI02 Caco-2 cells vitro determined effect its quantity time immunocompromised rat model. The results showed all five (fructo-oligosaccharides (FOS), galacto-oligosaccharides (GOS), inulin, stachyose, xylo-oligosaccharides (XOS)) significantly promoted L. DALI02. It was found 0.5% (w/w) inulin had best promotion effect, FOS strongest (the rate increase by 1.75 times). In addition, 0.05% FOS, 0.20% GOS, 0.30% XOS from 1.72% 3.98%. After 1 w intervention, fermented broth with group higher than group. intervention extended d 4 w, amount increased 4.32 lgcopies/g 5.12 lgcopies/g. serum levels lipopolysaccharide (LPS) D-lactic acid rats were reduced, most significant group, LPS 74.11 pg/mL 40.33 μmol/L, respectively. Complex residence strain intestine, which helps restoration intestinal barrier function other probiotic effects.